Extraction of Methadone & Imipramine from Serum Using Immobilized Liquid ExtractionTM (ILE) Well Plates
ثبت نشده
چکیده
The following application note demonstrates the ability of Immobilized Liquid Extraction Well Plates to provide high-throughput bioanalytical extractions of methadone and imipramine directly from serum. ILE Well Plates exhibit highly efficient (99.6%) and reproducible (± 0.6 – 4.1% RSD) extractions for a wide range of analyte concentrations (10 – 20,000 ng/mL).
منابع مشابه
Comparison of the Modified QuEChERS Method and the Conventional Method of Extraction in Forensic Medicine to Detect Methadone in Post-Mortem Urine by GCMS
Background:Extraction of drugs is one of the biggest concerns and the most important part of preparation and determination in forensic medicine. The lack of an easy, efficient and fast extraction method is the most important and most difficult problem despite the development of forensic centers and their being equipped with new diagnostic devices. In the present study, a comparison was conducte...
متن کاملA simple and sensitive HPLC method for analysis of imipramine in human plasma with UV detection and liquid-liquid extraction: Application in bioequivalence studies
High-performance liquid chromatography (HPLC) methods employing ultraviolet (UV) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. Therefore, in the present study a simple, rapid and yet sensitive HPLC method with UV detection was developed and validated for quantitation of imipramine in human plasma samples. An efficient ...
متن کاملSample preparation and liquid-chromatographic analysis for tricyclic antidepressants in serum.
We report two separate sample-preparation procedures and a "high-pressure" liquid-chromatographic method for quantitating nine tricyclic antidepressant drugs: trimipramine, doxepin, amitriptyline, imipramine, desmethyldoxepin, nortriptyline, desipramine, maprotiline, and protriptyline. The drugs are extracted from 1 mL of serum by a single manual hexane extraction or by using DuPont's PREP I Au...
متن کاملAnalysis of drug-protein binding by ultrafast affinity chromatography using immobilized human serum albumin.
Human serum albumin (HSA) was explored for use as a stationary phase and ligand in affinity microcolumns for the ultrafast extraction of free drug fractions and the use of this information for the analysis of drug-protein binding. Warfarin, imipramine, and ibuprofen were used as model analytes in this study. It was found that greater than 95% extraction of all these drugs could be achieved in a...
متن کاملA Sensitive Electrochemical Sensor for Determination of Imipramine in Urine Sample Using Carbon Ionic Liquid Electrode Modified With Montomorillonite Nanoclay
We used an effective electrochemical sensor for the determination of imipramine at pH 7.2 using a carbon nanocomposite electrode. The electrode has been designed by incorporation of montmorillonite nanoclay into the carbon ionic liquid electrode. The high sensitivity of 1.714 μA (μM)-1, two linear calibration ranges of 0.1–2 μM and 2-40 μM, and detection limit of 19 nM were achieved. The relati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2007